A great reference for the Bio-IT community. We have all been doing white paper marketing and marketing via article publishing. A step in here is this qPCR technical guide. qPCR or real time PCR is one of the most commonly used molecular technique for identification of a target gene. The technique is accurate, sensitive and less time consuming. The dependability of results is high as compared to hybridization based techniques such as northern blotting etc.

The technical guide on qPCR contains each and every detail related to the qPCR experimentation starting from a technology introduction to primer and probe design, instrumentation, application guide, optimization and troubleshooting sections.

Check it out on the Sigma-Aldrich website at:
http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Molecular_Biology/PCR/Product_Lines/Quantitative_PCR/qPCR_Technical_Guide.html